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Note that we explore here the minimal model that contains just one type of activator of each type of curvature (concave and convex), while in the real cell many different proteins of both curvatures coexist and may play a role in CDR formation, as we indicate in Text S1.
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These modifications are indicated in Text S2 and the modified matrix is given in Text S3.
Follow-up experiments, as indicated in text, were carried out with green or red-ripe tomatoes of cvs.
Molar concentration of Ash2L, RbBP5/Ash2L and MLL1SET proteins for each reaction were indicated in text or figure legend.
All other chemicals, for which any supplier has not been indicated in text, were purchased from Sigma-Aldrich Sweden, Stockholm, Sweden.
"Match" and "No Match" were indicated in text using blue and orange text boxes respectively to allow ease in scoring using the "Slide Sorter" option of PowerPoint.
T cell clones (TCCs) were grown in complete RPMI medium supplemented with 5% human serum (RPMI-HS) and cytokines as indicated in text.
For plate assays, bacteria were seeded into soft LB or M9 agar (0.3% agar) with or without X-gal (40 µg/ml) as indicated in text.
Total RNA was prepared from cells at times and conditions indicated in text using the TRIzol reagent (GIBCO-BRL) according to the manufacturer's protocol.
On occasions (as indicated in text) images were collected and displayed as single-plane images.
Comparison between MM cases with (positive fold change) and without (negative fold change) chromosomal abnormality as indicated in text.
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