But, true to the cooler mood of Scandinavian TV, the domestic secrets in The Legacy are less startling, involving infidelities and, as the title indicates, final bequests and requests.
LiCl was added from a 8M stock solution to the indicated final concentrations.
Variations of PstPc activity by addition of Zn2+ and Pi was assessed by adding ZnCl2 or sodium phosphate [pH 7.2] to the reaction mixture as above, to achieve the indicated final concentrations.
The following inhibitors were used at the indicated final concentrations: ALK inhibitor (A8301, Tocris Bioscience; 0.5 µM) and JAK inhibitor I (Calbiochem 420099; 0.6 µM), SU5402 (Calbiochem 572630, 10 µM).
Suitable Hb concentrations for calibration were obtained by diluting the concentrated lysate with solution A. Free calcein was added to the Hb samples from the 10 mM stock in DMSO to render the indicated final calcein concentrations.
Valine (150 mg/l), leucine (100 mg/l), isoleucine (30 mg/l), adenine (20 mg/l), histidine (20 mg/l) or uracile (20 mg/l) were added at the indicated final concentrations when required.
For each experiment, cross-linkers were freshly prepared as a 20 mM solution in Dimethyl Sulfoxide (DMSO) and diluted to the indicated final working concentrations in Phosphate-buffered saline, pH 7.4 (PBS, Fisher Scientific).
Agar plates containing experimental treatments were prepared from the same batch of NGM agar as the control plates except that the respective chemical was added to obtain the indicated final concentrations from a sterile stock solution (10 µM each).
When necessary, the following antibiotics were added to the indicated final concentrations: kanamycin (Km), 50 µg/ml; gentamicin (Gm), 3 µg/ml); carbenicillin (Cb), 50 µg/ml, nalidixic acid (Nal), 5 µg/ml; and ampicillin (Amp), 50 µg/ml.
