Comparing the median fluorescence for the populations in Figure 2, A C, the flow cytometry indicates expression from the pCU-EGD2-GFP is the lowest (median ∼30), followed by HHT2 (median ∼89), and strains carrying pCU-PDC1-GFP had the highest level of fluorescence (median ∼202).
The ratio of the dominant splice isoforms of mrps16 (i.e., circular, linear, and lariat precursor) is consistent between the genomic and plasmid copy of the gene, indicating expression from the plasmid does not bias the splicing pattern in a significant way.
Colors indicate expression levels of DEGs.
Previous analysis of ESTs derived from cotton stems indicated expression of genes associated with lignin biosynthesis increased as stems matured [ 8].
Red staining indicates expression of RAGE, yellow staining indicates ZO-1 expression, and green staining indicates expression of SP-D.
Together, these results indicate that expression from the MIR168a promoter is stronger and broader than that from the MIR168b promoter.
These data indicate that expression from the 35S promoter, like that from the DIR1 promoter region, is reduced in response to inoculation with Pst.
A -1 value in the (Populus 12 hours) - (Populus 37 hours) column would indicate that this gene had 2-fold higher expression in the C. thermocellum Populus 37-hour fermentation compared to the C. thermocellum Populus 12-hour sample; a positive value would indicate greater expression from the Populus 12-hour sample.
While RT PCR analysis using cDNA isolated from mouse adult tissues and fetuses (E7, E11, E15 and E17) also failed to show Hmgpi expression, RT PCR analysis for preimplantation embryos indicated Hmgpi expression from the 2-cell embryo to the blastocyst stage (Fig. 2A).
The resulting network was then overlaid with expression ratios (red colored nodes indicate over-expression and blue colored nodes indicate under-expression) from our MPSS analysis of GBM tissues and normal brain tissues.
