Sections (5 µm) were stained with haematoxylin and eosin, periodic acid Schiff's, Masson's trichrome or silver methenamine histological stains as indicated, and assessed in a blind manner.
The rate of decline in FEV1 % predicted has been studied in CF patients to better understand the progression of lung disease, in order to identify high-risk groups in whom aggressive therapy might be indicated and to assess therapeutic interventions.
Mice were bled as indicated and serum assessed for IL‐22 by ELISA (eBioscience, San Diego, CA, USA).
As a consequence, it is not known, which features of the autoantibodies should be assessed to indicate and control antibody-directed therapy.
These findings indicate that assessing and intervening for the psychological impact of the infectious outbreak on HCWs, especially on nurses and auxiliary staff, is of particular importance in planning for the current and future outbreaks of infectious diseases.
Plates were then incubated for a period of time as indicated and cell viability assessed using the MTS assay as per the manufacturer's protocol.
After 24 hours, cells were treated with E2 as indicated and cell viability assessed after 6 days using the Cell Titer-Glo luminescent cell viability assay according to manufacturer's instructions.
A number of disease conditions are known where precautions for the use of NRT products are indicated, and will be assessed in each participant by researchers prior to randomisation.
Cells were stimulated or not with TGF β as indicated and apoptosis was assessed using MTT (3- 4, 5-Dimethylthiazol-2-yl -2, 5-Dimethylthiazol-2-yl -2mide) 5-Dimethylthiazol-2-yl -2ell as calcein-AM (calcein-acetoxymethyl ester) assay, a more sensitive assay for early apoptosis detection.
