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Results show a reduction of approximately 50% in both mutant strains and clearly indicate a defect in nuclear oscillations, most probably linked to defective cMT functions.
These results indicate a defect in the migration of renal embryonic endothelial cells that may cause defective glomerulogenesis (Fig. 3A).
Prolonged mealtime and increased circulating ghrelin indicate a defect in meal termination mechanism.
All these phenotypes might indicate a defect in repair of DSBs resulting from intrinsic sources such as DNA replication.
To address this question, we isolated total RNA from the eyeballs of RP18 knockin mic[28], [29] and measured mRNA levels of several retina-specific genes (including photoreceptor-specific genes) since a decrease in levels of mRNA may indicate a defect in RNA splicing.
These findings, together with the aberrant tendency for swollen knobs to protrude above the ciliary layer in the MKS3 mutants, indicate a defect in the maturation process of OSNs, and consequently a flaw in either the initial organization, or in the maintenance, of the OE.
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Strains defective in Suv3 or Dss1 show accumulation of mitochondrial mRNAs, indicating a defect in their turnover (Dziembowski et al. 2003; Malecki et al. 2008).
In addition, one dark spot marked by a square only exists in the middle subcell, indicating a defect located in the GaInAs subcell.
Moreover, the tight junction protein ZO-1 is mislocalized in the tal1-deficient endocardium, indicating a defect in intercellular junction formation.
The resulting virus particles have a regular protein composition and no changes in their morphology were obvious by electron microscopy, but their hemolytic activity is reduced indicating a defect in membrane fusion.
With PFOA exposure, cytosolic PKC concentrations were significantly elevated for all three isoforms; despite the overall increase in PKC expression, membrane-associated PKC was unaffected, indicating a defect in PKC translocation.
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