We then used cutoff values of 0.1 - 1% the frequency of the index sequence to indicate probable mis-sequencing events.
cDNA fragments were purified separately from each sample and indexed with unique nucleic acid identifiers (Illumina TruSeq V2 index sequence).
For indexed sequencing libraries [ 9, 10], such clusters are efficiently removed by an index sequence filter step.
Each sample was distinguished by an index sequence of second PCR primers following PCR amplification.
Index coder ζ: I → { bitstream }, which transforms the index sequence generated from encoder ϕ to a bitstream.
Suppose is a bounded sequence of real numbers and is a double index sequence of real numbers which satisfy. for each.
We use the 2-D Viterbi decoding based on (15) to detect the most likely Gaussian index sequence and then estimate a soft mask of the target speaker using the MAP estimator in Section 2.2.
In order to eliminate the influence of seasonal factors in the index sequence, we introduce the X-13 Census method to adjust the two variables to make the sequence more realistic and objective.
Suppose { r k } is a bounded sequence of real numbers and { d k, l } is a doubly index sequence of real numbers which satisfy lim sup k → ∞ lim sup l → ∞ d k, l ≤ 0, and r k + l ≤ r k + d k, l. for each k, l ∈ N. Then lim k → ∞ r k = a for some a ∈ R. Lemma 2.7[30, 31].
Errors in these index sequences could be a potential cause, when the species identified by the tag sequences does not correspond to the species represented by the index sequence.
With regard to the responses seen in patient OP428 targeting the HLA-B8 restricted EL9 epitope (Table 5), we found that 0.8% of the CTLs were actually directed at the emerging E93D mutant epitope (93DVKDTKEAL101) at week 63, while there was no recognition of the index sequence, 93EVKDTKEAL101.
