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Ten thousand permutations of individual genotypes among samples (either between or within groups), or whole samples among groups, tested the significance of each index of differentiation.
The latter is a combined index of differentiation status, nuclear pleomorphism reflecting genomic instability and number of cells in M phase (Elston and Ellis, 1991; Rampaul et al, 2001).
The maximized index of differentiation among groups, analogous to an F-statistic (Wright 1965), was estimated using a distance matrix that was previously generated in PAUP (Swofford 2002) using a GTR nucleotide substitution model (Tavare 1986).
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Morphological analyses were performed to determine myotube number, diameter (μm) and myonuclear accretion as indices of differentiation and myotube hypertrophy.
Median values of the indices of differentiation were compared by a Kruskal Wallis nonparametric ANOVA KWW test).
A linear regression between patch areas (expressed as log km) and different genetic indices of differentiation (mean pairwise D EST and mean pairwise F ST ) and diversity (allelic richness A R and expected heterozygosity H E ) for each SL were also performed to assess the effect of confinement within small enclosed protected areas (details for each SL are displayed in Table 1).
An index value of differentiation among populations greater than 0.25 is considered high, and Index values greater than 0.5, mean that populations are so different that they could even be in the process of speciation, which is not supposed to be the case with J. curcas [ 54].
Osteogenic differentiation at day 21 and day 35 was quantified by the index of osteogenic differentiation (IOD IODOD = optical density of differentiated samples optical density of controls Chondrogenic differentiation of P3 MSCs was performed in a 3D-pellet culture system.
Previous studies reported various index values of differentiation among populations [ 18, 24, 54– 58].
This involves regression of an index of genetic differentiation on marine geographic distance among pairs of individuals (see Methods).
A common index of genetic differentiation is FST, which measures the fraction of overall diversity that occurs among populations.
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