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Pseudo-singletons refer to the de novo assembled Illumina sequences that were not assembled into contigs during the Gene Index Assembly process.
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The idle time is the most interesting performance index for assembly line design.
The proportion of sequences contributing uniquely to a contig was used as an index of assembly success.
We adopted two groups of index for assembly evaluation according to an earlier study [ 38].
After indexing the assembly contigs, we filter the paired reads for those that anchor uniquely in the assembly, then use Bambus [15] with a minimum threshold of 5 paired read links to join two contigs into a scaffold.
Under these conditions, presence of the normally retained subunit on the cell surface can be used as an indirect index of protein assembly in the ER.
Only filtered reads and reads mapped to contig ends went into the de novo assembly index.
The aim is to optimize some performance indices of an assembly line.
Some general quality indexes of this assembly are listed here: coverage of the genome after mapping and assembly (63.2%), number of scaffolds (1,612,870), and N50 scaffold length (2092 bp).
The following results were obtained with a rigid probe based on a Graded-Refractive-INdex (GRIN) lens assembly with a diameter of 2 mm and a length of 150 mm (GRINTECH, Germany).
Measurement of actin binding domain fused GFP (ABD-GFP) expression was used as an index of F-actin assembly and localization [25] (Fig. 5A).
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