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As for the previous study, the present investigation of slender column elements is based on the use of indeterminate specimens (which represent more realistically elements in a building than their determinate counterparts) designed by using two different methods: the truss analogy method, as applied by current codes of practice, and the compressive-force path method.
After specimens were categorized as non-pregnant from the pregnancy-unknown category and approximately 18% (21/119) of repeatedly reactive EIA and Western blot-negative and 35% (20/57) of indeterminate specimens were reclassified because they may have represented true infections, the false-positive rate among non-pregnant persons was 0.16%.
Repeat testing of indeterminate specimens resulted in conversion to negative in 51% of cases and positive in 20% of cases (Table 2).
Repeat testing of indeterminate specimens would be associated with increased cost and may not significantly improve performance characteristics as it produced mixed results in our cohort.
One strategy is a "public health approach" that incorporates routine screening of all HIV antibody negative or indeterminate specimens into pools which are then tested for HIV RNA (Flanigan and Tashima 2001; Quinn et al. 2000; Pilcher et al. 2005).
This algorithm diverged from the above protocol in that third-generation EIA-reactive, Western Blot-negative or indeterminate specimens were subjected to an individual HIV-1 NAAT (COBAS Ampliprep/COBAS TaqMan HIV-1 Test, v1; Roche).
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Each indeterminate specimen was subjected to repeat PCR testing using Xpert® on an aliquot of frozen (−80°C) stool to assess whether repeat testing of indeterminate results could improve detection of C. difficile compared with toxigenic culture.
In the remaining 69 WB-indeterminate specimens, we could not rule out exposure to SIV from nonhuman primates, from handling infected animal meat before consuming it, or from keeping monkeys as pets.
To determine whether HIV- and SIV-like RNA could be detected in the 70 WB-indeterminate specimens, we attempted reverse transcription polymerase chain reaction (RT-PCR) amplification of HIV-1, HIV-2, and SIV.
However, when malignant (positive) cytology testing and the results of mutational profiling on negative, atypical or indeterminate cytology specimens were analyzed together, 57% (12/21) of aggressive cases were detected (Table 4).
Of the 70 WB-indeterminate serum specimens, 69 showed a broad range of HIV-1 WB-indeterminate band patterns, and 1 had an HIV-2 WB-indeterminate pattern with weak reactivity against gp36, p68, and gp80 proteins.
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