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These produced one female sample and one sex indeterminate sample.
HCV infection was defined as a positive or indeterminate sample (EIA test) and confirmed HCV-RNA technique.
As for an indeterminate sample for HIV infection in the IHEs, HIV infection had to be confirmed by the division of AIDS of Korea Centers for Disease Control and Prevention (KCDC) [ 7, 8].
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Indeterminate samples (n = 24) were classified as negative for analysis.
Assays sensitivities were not affected by addition of indeterminate samples since we included these samples as HIV negatives (Table 3).
While there was close correlation between the reaction patterns of WB and OIC-HIV for the positive samples, there was considerable variability in the negative and indeterminate samples.
Interestingly, all attempts to identify proviral HIV DNA in these 44 indeterminate samples using universal and broadly sensitive PCR primers were unsuccessful.
The procedure of Cattamanchi et al. was followed in that indeterminate samples for which a majority of novel bands appeared for the post-treatment infection were scored as new infections [21].
Indeterminate samples detected by EIA were excluded.
Meanwhile, indeterminate samples are challenging for clinicians and researchers.
This analysis excluded 16 indeterminate samples by the UW-WB and considered 10 indeterminate samples by Kalon as negative.
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