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For each combination of developmental stage and IP factor, three biological samples were profiled by ChIP-chip, and one of these samples was independently profiled by ChIP-seq.
Several studies have independently profiled fluorescence-activated cell sorted (FACS) purified normal mammary cell types from both human [ 19– 21] and murine [ 22, 23] mammary tissues.
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To begin to understand how epigenetic regulation influences CNS cell function, and because so little is known about the expression of key Jumonji demethylases in any CNS cell type in the adult, we used the present method to independently profile the expression of the 26 best characterized Jumonji histone demethylase family members in microglia, neurons and astrocytes.
Replicate pools of extreme lines can be profiled independently, so that differences in gene expression will be specific to the differing pools.
This notion was further supported by examining the locations of cells cultured and profiled independently in the transcript profile space: P19 EC cells before (P0) and after 4 days of RA induction (P4); NS cells derived from adult mouse brain and their differentiated cells (DC); and TS cells and E12.5 PL.
We present an automated approach, 'AMY-tree', which is able to determine the phylogenetic position of a Y chromosome using a whole genome SNP profile, independently from the NGS platform and SNP calling program, whereby mistakes in the SNP calling or phylogenetic Y chromosomal tree are taken into account.
M-OCORS with a release profile featuring exposure of the intestine was profiled in two independently performed studies demonstrating preventive effects in chemically induced colitis.
The training and the validation cohorts were profiled separately and independently.
The profile derived from this panel can be compared to an independently genotyped profile for the same individual, allowing accurate validation of data and sample pairings, at a modest cost per sample.
However, comparing the profile affiliation across groups is not straightforward since (1) the CAT model infers profiles independently from each clade resulting in different sets of profiles, (2) the number and nature of profiles varies during the MCMC, and (3) different profiles can be affiliated to a given site during the Monte Carlo Markov Chain (MCMC).
The MD statistic will therefore be different from zero when there is a change in the methylation profile, independently of a change in the coverage profile.
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