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Cy5 and Cy3 fluorescent intensities were corrected independently for background signal and normalized using specialized software (BeadStudio; Illumina).
Cy5 and Cy3 fluorescence intensities were corrected independently for background signal and normalized using GenomeStudio software (Illumina, Inc .. Continuous β values that range from 0 (unmethylated) to 1 (methylated) were used to identify the percentage of methylation, from 0%to100%0%, for each CpG site.
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Shadow detection which was often done independently of background subtraction is integrated in our approach.
MIM allows individual QTLs to be detected independently of background noise and of previously detected QTLs.
"Background" was defined independently for each spot from its surround and subtracted from each spot value.
We extend this method to a local background, which is computed independently for each voxel in the spill-out region.
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