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This approach, although flexible because it allows independent integration of new annotation sources, presents scalability limitations and does not integrate well with existing command-line tools.
Therefore, the super twin T-DNA vector (pTRIDT313-g) exhibits independent integration, heredity, and normal gene function from two T-DNA cassettes.
Sequence analysis of 196 independent integration events derived from three cell lines revealed ∼101 integration sites: 56% of the events were recurrent integrations distributed among 19 pseudo attP sequences.
Generally, at least two independent integration lines were tested for each of the endoA constructs.
These GRD elements have arisen through at least two and possibly three independent integration events during the evolution of these four Nicotiana species [26], [28], [31].
The fact that two independent integration methods (ode45 and ode15s) yield consistent results enhances my confidence in these findings (Figure S4).
Similar(38)
There were multiple independent integrations in several genes, including MKL2 and BACH2; many of these integrations were in clonally expanded cells.
We also identified four independent integrations in the tumor suppressor gene Pten.
Notably, we found four independent integrations in the atypical protein kinase C (PKC) member Prkci.
Therefore several independent integrations, mainly from closely related hosts, have to be considered as an alternative explanation.
Our search of the chicken nuclear genome indicates the presence of 13 apparently independent integrations of mitochondrial DNA genes.
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