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Since there may be duplicate or overlapping contigs between the independent assemblies, Gentig is used to assemble all of the contigs together to remove any redundancy, yielding a set of seed contigs.
3) Assembled these sampled reads using the newbler assembler and mapped the trimmed contigs from these two independent assemblies onto each other to determine the precision, e.g., the differences that one obtains through sequencing and assembly errors when sequencing the same DNA.
The ADR consists of two mechanically independent assemblies.
There exists no measure that can compare the accuracy of these two independent assemblies because there is no reference genome to map them to.
In addition, two independent assemblies were performed using reads from each pool.
Similarly, independent assemblies were also performed for SF and BF libraries.
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Contig assembly takes place in three stages, grouping the reads first into regions smaller than a clone length for independent assembly, then combining the assembled contigs in increasingly larger regions.
For each library, an independent assembly was performed with the Newbler software, and the number of assembled contigs ranged within libraries from 7,800 to 14,008 with an average length of 831 1,148 nt.
"I'd put it more in terms of expansion" of the existing factory, rather than a second, independent assembly plant, said an auto industry manager who spoke on the condition of anonymity.
Mr McNarry, an independent assembly member, said he would not be asking that public money be used to "bail out a failing enterprise".
Enhanced DNaseI cleavages were observed at nucleotide positions 6 and 9 from the terminus on U3 but not on U5, suggesting independent assembly events.
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