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Indeed, insertion times decreased from 24 min in the first study of the device (8) to 15 min in this study.
Indeed, insertion of the HindIII site has such a negative effect on IRES function that detection of the IRES-controlled product can be difficult, and sometimes even below the levels of detection.
Indeed, insertion site selection during in vitro transduction could be driven by tethering of transcription factors (TF) to specific regions according to TF binding sites location (Felice et al, 2009) and seems dependent on cellular determinants as well as on vector design (Lewinski et al, 2006).
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We found that 95% of LTRs had a divergence between their repeats that was lower than the average divergence between mouse and rat, which suggests that the vast majority of LTRs, which we scored as new insertions, have indeed inserted since mouse and rat diverged.
Indeed, an insertion sequence IS1 is found in the promoter of the ymc operon in E. coli K-12 but not in pathogenic E. coli strains.
Indeed, membrane insertion of C2B occurs preferentially towards the PIP2-rich microdomains of the t-membrane.[34], [35], [36] We propose that this model provides an energetically plausible means to instantaneously promote fusion (see Discussion).
Indeed, this insertion is located in the first intron of maxillopedia.
Indeed, such insertion would most likely not produce valuable structural information.
Indeed, the insertion of hydrophobic parts of a protein into a membrane is one of several mechanisms by which proteins can deform membranes (McMahon and Gallop, 2005).
Given their abundance and their capacity to act as mutagens, it would be strange indeed if insertion of TEs never created adaptive changes.
Indeed, an insertion of an Ω loop was observed located at the interface between the Ig2 and Ig3 domains of human CNTN5 and -6, and predicted to be flexible (supplementary material Movie 1).
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