Generating and genotyping individual knockout clones for gsk3, tkl3, and PBANKA_082960, a putative protein kinase of unknown function, confirmed the barseq phenotype and established that these genes are indeed dispensable for blood stage growth.
Furthermore, depletion of ER glutathione was without effect on two sensitive, broad-spectrum UPR reporters, arguing that glutathione is indeed dispensable to a broad range of processes required for protein folding homeostasis in the ER of cultured mammalian cells.
Indeed, despite dispensable for SFV fusion which occurs in Rab5-positive endosomes, enrichment with Rab7 and subsequent migration of the virus contained in these Rab7-enriched domains along microtubules was reported for SFV [52].
Indeed, eEF1γ appears dispensable for translation, its absence doesn't seem to affect global rate of translational elongation.
Indeed, telomerase activity is dispensable for transformation of cells with long telomeres (Seger et al, 2002), and studies with telomerase inhibitors indicate that they are effective preferentially in cells with short telomeres (Hahn et al, 1999; Herbert et al, 1999; Wang et al, 2004; Brennan et al, 2010; Wu et al, 2012; reviewed in Buseman et al, 2012).
Indeed, ENO1 appears to be dispensable in tachyzoites: cells with a targeted deletion of the gene grow well in vitro and in vivo, but cyst numbers in chronically infected mice are significantly decreased [ 15].
Indeed, an annotated super-enhancer is dispensable for NEK6 overexpression and for maintaining the architecture of a B cell-specific regulatory hub.
Indeed, the Flv2 and Flv4 proteins are dispensable under HC conditions, at least when the stress conditions induced by high light are not too severe (Figure 7).
Indeed, Set2 activity in germ cells was dispensable for bam expression (supplementary material Fig. S2).
Indeed, whilst Drp1-dependent mitochondrial fission may be dispensable for non-polarized cells, it is crucial for extremely polarized cells such as neurons, where mitochondria must be shaped into small enough entities capable of distributing throughout narrow neurites where they are essential to assist synapse formation and proper function (Fig 1).
Indeed, we found that RIPK1 and RIPK3 are largely dispensable for colon cancer cell death induced by many common chemotherapeutic agents.
