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Indeed, after application of 150 mM NaCl, we observed visible signs of stress including loss of turgor.
Indeed, after application of 42 °C for a longer time or hydrogen peroxide stimulation, Mss4 was transiently upregulated at both mRNA and protein levels and half-life of its mRNA was prolonged compared with unstressed cells.
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Indeed, after the application of 1400 W (a synthetic blocker of inducible NOS) in a pig endotoxemia model, microvascular perfusion was restored by a redistribution within the gut wall and/or an amelioration of the cellular respiration [ 60].
Indeed, the Mss4 promoter activity was significantly reduced after application of all stress stimuli used but not by growth stimulating factors.
However, at least some of the effects we observed on entire Echinococcus larvae after application of HNMPA(AM 3 could indeed be due to inhibition of EmVKR rather than EmIR1 and EmIR2.
Fig. 5B shows the increase in fluorescence in horizontal cells after application of 25 mM acetate, confirming that acetate indeed leads to a decrease in the intracellular pH.
Indeed, we also detected free cytosolic Ca2+ shifts in our assay after application of ATP in a similar setting as before (EC25 and EC75).
Indeed, the results of the present study have shown that BIC and BD values seemed to be ameliorated after application of rhBMP-2 at far lower concentrations.
Yet, application after application, interview after interview (five within the last three months) I get the same response: "Lots of quality applications, not successful this time".
Indeed, its application in the production organizations was examined.
Recruiters and hiring managers with job openings must filter through application after application, resume after resume.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com