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After 1 h of incubation (room temperature), PE detection reagents were added and incubated for 2 h, the samples were then washed, resuspended and analysed with a flow cytometer (BD LSR-II) and the FCAP Array software.
The Mycopolitan team erected two hoop houses in the basement a heated incubation room, and a cooler humidity-controlled grow room.
They share a common heat pump unit that delivers chilled water to the incubation room, and the makeup water is from several wells on site.
All the lamps were also turned off in the incubation room and noises were minimized.
First new technologies require a "protected space," a niche, which acts as an "incubation room" in which actors form relationships and may learn and shape their expectations.
Electric fans were used 4 h/day during incubation for basidiocarp formation to provide homogenous ventilation condition in the incubation room.
Similar(42)
Primary antibodies were incubated overnight at 4 °C, followed by 1 h incubation at room temperature in secondary antibody.
After 15 min incubation at room temperature, the column was washed with RA2 and RA3 buffers.
Reactions were observed after incubation at room temperature for 30 min.
After 10 min of incubation at room temperature sulfite was detected at 670 nm.
After incubation at room temperature for 1 h, the samples were washed three times with PBS solution.
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