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Following incubation, agar was removed and the plates were stained for 5 min with 0.5 % crystal violet.
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We found that the bacteria from the shrimp degraded AMP to AdR (Table 2; Fig. 2) and that the bacterial numbers increased after 2 days of incubation on agar plates with 1% NaCl at 20 °C (Fig. 6).
After 7 days of incubation, the agar matrix was solubilized and cells stained and absorbance was recorded at 570 nm.
After 10 days of incubation, the agar matrix was solubilized, and 3- 4,5-dimethylthiazol-2-yl -2,5-diphenyl tetrazolium bromide was added to each well.
After overnight incubation the agar plates demonstrated numerous small colonies with a dark violet metallic pigmentation.
After incubation, ALOA agar plates were examined for typical L. monocytogenes/L.
Wild-type Sz. japonicus cells form hypha under prolonged incubation on agar media where nutrients are limited.
The thermograms of T. versicolor in untreated and modified beech wood (Fig. 3a) displayed typical courses of heat production after 3 weeks of incubation on agar plates.
This cut-off was based on testing the same amount of soil as used for incubation of agar plates dissolved in 200 μl sterile water.
After 24 h of incubation, an agar block (~8 mm in diameter) covered with 5-day-old fungal mycelium was excised and placed onto the PDA-filled section.
The CoNS samples tested showed negative test results both when tested directly on blood material and on colonies after incubation on agar plates (n = 20).
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