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After incubation with primary antibodies, membranes were incubated with a peroxidase conjugated secondary antibody (Jackson Immunoresearch).
Embryos were then incubated with a DIG or Fluo labeled probes overnight.
The slides were then incubated with a fluorescent secondary antibody (1 200 dilution; Invitrogen).
Immunoblots were incubated with a specific primary antibody and a horse radish peroxidase-conjugated secondary antibody.
The membrane was washed with TBST for 5 min and incubated with a primary antibody.
CM was collected, as described above, and incubated with a 1X general protease inhibitor.
Whole L. monocytogenes were incubated with a library of single-stranded DNA (ssDNA) aptamers.
Hybridized embryos were washed in a formamide series and incubated with a secondary antibody overnight at 4°.
The blots were incubated with antibodies for albumin (CST, # 4929S), washed, and incubated with a horseradish peroxidase-conjugated secondary antibody.
The cell lysate was incubated with a final concentration of 2% (w/v) DDM for 1 h at 20 °C.
Columns 4 6 were incubated with a pre-immune serum and columns 7 9 with the anti-rEnStef serum.
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