Sentence examples similar to incubated the primary from inspiring English sources

To prove specificity of the antisera, we either re-placed the primary antibodies with normal rabbit serum (DAKO Diagnostic) or we pre-incubated the primary antibody with recombinant meprinβ (competitive inhibition) [60].

Next, cells were incubated with the primary antibody at appropriate dilution and incubated overnight at 4°C with gentle rocking.

Cover slips were incubated with the primary antibodies for 1 hour at room temperature or overnight at 4°C.

Membranes were incubated with the primary antibody overnight at 4°C.

The membrane was incubated with the primary antibody followed by HRP-conjugated secondary antibody.

The blots were incubated with the primary antibody overnight at 4°C.

Briefly, the sections were incubated with the primary antibody overnight at 4°C in a humidified chamber.

The sections were then incubated with the primary antiserum overnight at 4°C.

As a negative control specimens were incubated without the primary antibody.

Some tissue samples were incubated without the primary antibody or unrelated IgG, as negative controls.

The blots were incubated with the primary antibody and subsequently with the horseradish peroxidase (HRP) conjugated secondary antibody.