To optimize the effective concentration and incubation time for labeling, canine MSCs were incubated for different time periods (2, 4 and 6 h) with various nanoparticle concentrations (100, 200 and 400 μg/ml).
For this, the cells were seeded in 6-well plate, and after overnight incubation, the cells were exposed with PLGA and PLGA-LHRH formulation and incubated for different time points.
Post infection, cells were washed thrice with sterile 1× PBS to remove non-internalized virus and were incubated for different time periods in serum free media.
Cells were seeded in 96-well plates, transfected with siRNAs (see above) and after 48 h irradiated with UV-C (10 J/m2), and incubated for different time-periods (0 30 h) to allow RNA synthesis recovery.
e Panc1 cells were incubated for different time points with 0.5 mM of AGuIX.
To estimate the pH stability of KerP, the enzyme was incubated for different durations at 4 °C.
The culture medium was removed and replaced with fresh media containing GCN and FGCN and incubated for different intervals.
Cells were incubated for different time interval (0, 1, 2, 3, 4 and 5 h) in the presence of silica-coated CdSe QDs.
To this end, we analyzed the samples incubated for different amounts of time at an elevated temperature in SDS-PAGE and Western blot.
The mBSA incubated for different time intervals was analyzed in gel, and was visualized under an ultraviolet light (F), the reaction conditions were identical as those used for panel B. M, marker.
The mixture was then incubated for different time duration at ambient temperature, followed by centrifugation at 5000 rpm for 5 min. FITC was quantified, using a UV-vis spectrophotometer, according to its absorbance peak at 488 nm.
