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In the latter part of this paper, we explore this mapping relationship in the non-model species case of using increasingly evolutionary divergent species (from the target species) for grouping contigs by putative orthology (see section: Using increasingly divergent genomic reference species for RNA-Seq analysis).
Herbicide resistance evolution in weed species is a global challenge in modern agro-ecosystems and increasingly evolutionary concerns are raised for the sustainability of world food production in a changing climate (Neve et al. 2009; Powles and Yu 2010; Thrall et al. 2011).
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Recent studies have increasingly recognized evolutionary rescue (adaptive evolution that prevents extinction following environmental change) as an important process in evolutionary biology and conservation science.
Advances in computational hardware and software allow the testing and extrapolation of increasingly advanced evolutionary models and the development of the field of systems biology.
For the sake of continuity, however, we begin with progress coming from increasingly sophisticated evolutionary genetics (Maynard Smith 1998; Jobling et al. 2004).
These observations were extended to mammalian genes classified in four different ages -Old, Metazoans, Deuterostomes and Tetrapods-, which showed increasingly faster evolutionary rates in genes of more recent origin [ 6].
To validate our approach, we compared the performance of four increasingly complex evolutionary strategies available in D-Tailor at deriving full-factorial libraries for 30 different genes randomly selected in E. coli (Fig. 4A and B).
Over the last decade nematodes in the Caenorhabditis genus have come to serve as increasingly powerful evolutionary models for studying diverse processes ranging from the mutation rate [ 4- 6] to the evolution of reproductive mode transitions [ 7, 8] to speciation mechanisms [ 9- 11].
In any case, the use of the rarefaction approach to examine alleles private to combinations of populations can produce novel observations that augment those obtained from other methods of data analysis, and that can form the basis for hypothesis tests with increasingly complex evolutionary models.
To estimate the phylogenetic origin of sequences in the E. lineata transcriptome, protein lists were downloaded from NCBI using a series of scripts (Additional file 12) for a selection of taxonomic categories encompassing taxa of increasingly distant evolutionary relationship to E. lineata.
What appeared to be happening during the visit to "Explore Evolution" is that visitors' need-based explanations for the evolution scenarios became disassociated from the desire-based explanations and increasingly associated with evolutionary mechanisms of change.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com