Sentence examples for increasing transformation efficiency from inspiring English sources
Most progress in increasing transformation efficiency in coffee has been achieved by optimizing the production conditions of embryogenic cultures used as target tissues for transformation.
While this study has demonstrated both an increase in transformation efficiency and a decrease in plasmid-to-plasmid variation in efficiency, more effort could be spent increasing transformation efficiency.
We report here that treatment with LiAc and DDT before electroporation increased transformation efficiency to 225 ± 52.5 × 107 transformants per μg DNA, while with untreated cells or treated with LiAc alone transformation efficiency approximated 1.2 ± 0.5 × 105 transformants per μg DNA.
Since deletion of comI in B. licheniformis DSM13 resulted in twofold increased transformation efficiency by genetic competence and overexpression resulted in threefold decreased transformability, the function as a competence inhibitor became evident.
In another study, electrosprayed solution of plasmid containing gold nanoparticles (Au NPs) which were directed against bacterial cells increased transformation efficiency up to106 CFU/μg plasmid [6].
As increased transformation efficiency using unmethylated DNA is rare [28], the foreign genetic materials should be methylated prior to delivery to prevent cleavage by the RM systems.
Vacuum infiltration was performed three times to increase transformation efficiency.
Some proteins encoded by the vir regulon are non-essential for transformation but are known to increase transformation efficiency.
Significant trait improvement via biotechnology e.g. [ 69, 73, 74] with increased transformation efficiency [ 50] has been demonstrated in switchgrass.
In conclusion, the gene targeting system developed in this study significantly increases transformation efficiency as well as homologous recombination efficiency and omits the use of Δku70 strains.
We have shown that ARP6 is a negative regulator for the AMT process as knockout of ARP6 consistently and significantly increased transformation efficiency.
