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They require many steps or specialized equipment increasing the assay duration and cost.
Haraya et al. [74] confirmed that as the number of lysine residues increasing the assay would be more sensitive.
Further, co-trapped laser-cooled atomic ions sympathetically cool all the ions, increasing the assay phase space density and thus the mass resolution.
New pre-analytic approaches aim at increasing the assay sensitivity through enrichment of microbial DNA from blood samples.
Furthermore, increasing the assay period for up to 4 h resulted in no increase in OD above background levels for this enzyme (Supplementary Figure S4C).
Using a M. bovis BCGMtbNar recombinant strain, we could stimulate anaerobic respiration, increasing the assay window to a range compatible with high-throughput screening.
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Two affiliative aptamers were used to increase the assay specificity through sandwich recognition.
Hence, we have designed an alternative protocol to traditional enzymatic digestion of DNA which (i) limits the potential for artifactual oxidation, (ii) speeds up the assay markedly, (iii) increases the assay's sensitivity moderately, and (iv) addresses criticisms that have been raised concerning the efficiency of DNA digestion by nucleases.
This indicates that decreasing the plasma volume in samples increases the assay sensitivity.
The problem of using such a kit, however, is that it would increase the assay cost and turn-around time.
We then assessed which combination of these markers could increase the assay sensitivity without substantially lowering its specificity.
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