The rising heat of the abolitionist controversy and the consequent increasing stringency of slave laws in Southern and border states at length drew her into the public arena.
We have found it necessary to take measures of increasing stringency, not only against enemy aliens and suspicious characters of other nationalities, but also against British subjects who may become a danger or a nuisance should the war be transported to the United Kingdom.
These are, in order of increasing stringency, intelligibility, accessibility, and shareability.
(3) All types of policy instruments face challenges in design and implementation: understanding the selection impact of the chosen instruments, implementing increasing stringency levels, committing to an appropriate scale, and safeguarding policy stability.
The mix of power-generating sources in the United States has shifted away from coal in recent years, due to the increasing cost-effectiveness of renewable energy sources such as wind and solar, the decline in natural gas prices, and the increasing stringency of regulations on conventional pollutants.
The signals decreased with increasing stringency, resulting in melting curves that could be used to evaluate assay performance (Fig. 2B).
One would expect that additional cycles of molecular evolution in GFP insertion vectors with increasing stringency would likely produce a more robust and soluble Rv2911 variant.
We selected soluble and full-length mutants of the recalcitrant Rv0113 protein using hybrid GFP insertion reporters with progressively increasing stringency during successive rounds of evolution.
Proteins of different solubility were extracted from brain in buffers of increasing stringency, using a slightly modified protocol previously described [24], [25].
The blot was washed in SSC at 60°C, with increasing stringency washes, ending with 0.1 X SSC, 0.1% SDS, at room temperature.
Increasing stringency by using the best similarity score (less ties) increases FN to 38% and reduces FP to 4% (Table S2), close to the values observed for RSD.
