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Adsorption is sensitive to the frequency and increasing step of the external voltage and different desorption rates of 0.003 and 0.027 arem2 are observed from cations and anions, respectively.
The melting curve analysis of the primers was conducted at 60 95 °C, with a temperature increasing step of 0.06 °C/s (five acquisitions per degree of Celsius) at the end of each run.
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The pop values are taken in the range of [10, 100] in increasing steps of 10, while (p_mathrm{c}) values are ranged from 0.6 to 1.0 by incremental steps of 0.1.
Table 4 presents the performance of the GA over different iterations, starting from 100 to 1000 in increasing steps of 100, with respect to the time measured in seconds.
Melting curve analysis was performed with temperature increasing steps of 0.08°C per second, ranging from 72°C to 95°C.
For each data set, nucleotide sites were resampled to generate bootstrap pseudomatrices of 100, 250, and until 5000 characters with increasing steps of 250 sites.
This cycle was followed by a melting curve analysis, ranging from 55°C to 95°C, with temperature increasing steps of 0.5°C every 10 s.
For comparison, we applied the most differentially expressed genes as determined by a t-test or AUC values to SVM, also in increasing steps of 5 genes.
The amplification process was followed by a melting curve analysis, ranging from 60°C to 90°C, with temperature increasing steps of 0.2°C every 10 s.
To verify the specificity of each primer pair, a melting curve analysis was performed ranging from 60°C to 95°C with temperature increasing steps of 0.06°C/s (5 acquisitions per °C) at the end of each run.
Artificial pacing was performed, by repeated intermittent sequences of pacing for 30 s, at increasing steps of 20 bpm, till the maximum tolerated BP fall, or the loss of 1 1 ventricular capture.
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