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Furthermore, the recovery of the activity concentrations within the physical boundaries of the spheres increased with increasing sphere volume.
Increasing the height of fall (or impact velocity) has a similar, but surprisingly weaker, effect to that of increasing sphere diameter.
The debonding stresses increased with decreasing sphere diameter, whereas the length to diameter ratio of the resulting matrix cracks increased with increasing sphere diameter.
Sphere clusters were located in both arterioles and small arteries and increased in size with increasing sphere concentration, resulting in increased absorbed dose inhomogeneity, which contradicts earlier modelling studies.
Willowson et al. showed a phantom sphere of 90Y radioconcentration 3.9 MBq/ml and diameter 37 mm to have a recovery coefficient of approximately 0.8, and a trend was observed for recovery coefficients to approach 1 with increasing sphere size [33].
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At a plating density of 1.5 cells/µl, CD133 expressing cells exhibited increased sphere formation (Fig. 6A).
LA-N-5 spheres were further studied and showed a verapamil-sensitive side population, relative resistance to doxorubicin, and CD133+ cells showed increased sphere formation and tumorigenicity.
We show N1-ICD transactivates SOX2 to increase sphere formation, ALDH1+ and CD44+CD24low+cells.
Free-floating sphere formation is common for NSCs and CSCs, and we often see increased sphere formation in arsenic-induced CSCs.
Interestingly, full length AVEN was able to significantly increase sphere formation in the presence of miR30a overexpression to levels close to control empty-vector levels.
Moreover, LFG repression resulted in reduced cell adhesion, increased sphere growth and enhanced migration, thus conferring a higher metastatic capacity to NBL cells.
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