Sentence examples for increasing origin from inspiring English sources
Further, down-regulation of HDAC3 mimics actions of the HDAC inhibitor suberoylanilide hydroxamic acid (SAHA, vorinostat) in reducing replication fork velocity and increasing origin firing at sites of replication, likely due to chromatin changes [ 77].
The c- Myc proto-oncogene has been shown to accelerate S-phase by inducing premature origin firing initiation and increasing origin density (Robinson et al., 2009; Srinivasan et al., 2013).
This is consistent with previous observations showing that DS-deletion in P3-ΔDS-47 (a P3HR1 strain) results in loss of ORC binding and origin activity at oriP and increased origin activation at additional distant sites [15], [30].
This activation of late origins is reminiscent of the increased origin firing observed when mrc1 was deleted.
Thus, both mcm4 and mcm5 PS1-hp mutants had an increase in plasmid loss with a clear MCM phenotype that was partially suppressible by increased origin dosage.
Accordingly, CHK1-deficient cells exhibit increased origin activation and reduced rates of fork progression that can be alleviated by counteracting origin firing though Cdk2 or Cdc7 inhibition (Petermann et al., 2010).
In plasmid loss experiments, only mutants in initiation such as in cdc6 (Hogan and Koshland 1992) or orc2 (Loo et al. 1995) are suppressed by increased origin gene dosage.
For example, studies in Drosophila have shown that tethering histone acetylases to chromatin increases origin activity [43].
The replicon length scales with increasing inter-origin distances and is therefore a readout of the distance between activated origins.
To verify whether Plx1 is required for DNA replication in more stressful conditions, we studied the role of Plx1 in DNA replication in the presence of a limited number of replication origins and increased inter-origin distance.
Inhibition of Chk1 may result in increased replication origin firing and reduced fork progression leading to increased regions of RPA bound ssDNA.
