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Abundance generally declined with increasing modification, but was quite variable.
Initiator concentrations were optimized and the crosslink densities shown to increase with increasing modification.
MDA-MB-231 cells incubated with aHNE and subject to click chemistry revealed a concentration-dependent increase in aHNE adduction of Pin1, with increasing modification observed as low as 2.5 μM.
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Accordingly, these findings provide an explanation of the sexual dimorphism and increased modification of female reticulation in dytiscids.
Increased production and accumulation of methylglyoxal (MGO), as well as increased modification of proteins by glycoxidation, are hallmarks of aging and diabetes.
A mutation acting in this way would then enable the host to increase modification or rescue without having to interfere directly with Wolbachia transmission or manipulation.
Moreover, MGO was also shown to modify the co-repressor mSin3A, which results in increased recruitment of O-GlcNAc-transferase, with consequent increased modification of the transcription factor Sp3 by O-linked N-acetylglucosamine [46].
Increased production of MGO, increased modification of proteins by glycoxidation and accumulation of damaged proteins are all hallmarks of aging and of a number of diseases, such as diabetes and cancer [1], [5].
[ 26] 5. Promoting the increased modification of LDL-cholesterol.
Conversely, induced DPAGT1 expression in MDCK cells leads to increased modification of E-cadherin with complex N-glycans and its reduced membrane association (Sengupta et al. 2010, 2013).
In these cells, urea induces ROS production, which increases modification of insulin signalling molecules by O-GlcNAc and reduces insulin-stimulated IRS (insulin receptor substrate), Akt phosphorylation and glucose transport.
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