Sequencing of wild-type and white cDNAs from embryos revealed splice variants in each background yet white cDNAs consistently lacked exon 2. RT-PCR using primers to detect coding sequence for the mature Edn3 peptide revealed initially low but increasing levels of transcript in wild-type embryos beginning during stages of neural crest migration, but no expression in white mutants (Fig. 1B,C).
Three members (alpha, beta and delta) of the CCAAT/enhancer-binding protein (CEBP) family of transcription factors had increased levels of transcripts in the older mice.
Finally, using RNA interference of ORC1/CDC6, we show that DNA replication functionally intersects with transcription because the absence of the initiator leads to increased levels of transcripts from the DGC boundaries and derepression of MVSG silencing.
In contrast, T regs) from patients with glioblastoma multiforme exhibit increased levels of transcripts involved in inhibiting host immunity.
Furthermore, increased levels of transcripts for Bcl2 and Bcl2l1 may affect the limited degree of apoptosis associated with knockdown of Eef1a2 expression.
Interestingly, only three peyote OMTs showed increased levels of transcripts in buttons compared to roots.
Increased levels of transcripts for pathway members including JAK3, STAT3, SOCS3, PTPN1, CDKN1A, RRAS and MAPK1 were observed in nephritis.
There were 7 genes that also showed increased levels of transcripts after 6 h of carbon starvation and these are discussed in Section 3.1.6.
Specifically, these 16HBE14o- cells had increased levels of transcripts from genes associated with repair and inflammatory processes (e.g., matrix metalloproteinases, chemokines, and glutathione S-transferase).
At the same time, increased levels of transcripts encoding the low- and high-density lipoprotein receptors, as well as SOAT-1, are observed.
