Sentence examples for increasing hybridisation time from inspiring English sources

Increasing hybridisation time generally increases the specificity of the hybridisation.

In a preliminary experiment it was shown that this cross hybridisation could be eliminated by increasing hybridisation stringency.

Then, the probes and hybridisation conditions were optimised using unrelated normal amniocytes, lymphoblastoid cells and lymphocytes from the carrier to increase hybridisation signal intensity and decrease background.

However, increased hybridisation temperature at 50°C or 55°C reduced cross hybridisation at least 5% and lowered general amount of positive signal to 60%and40%0% (respectively).

A recently developed label-free system might be a step in the right direction as it reduces the preparation and hybridisation time of samples and in parallel increases the sensitivity [ 59].

The arowana DNA biosensor response is also dependent on the DNA hybridisation time.

Fig. 8 Effect of temperature on the DNA hybridisation time of arowana DNA biosensor.

The assay was optimised with regards to methylene blue concentration, hybridisation time and regeneration.

A faster DNA hybridisation time could be achieved at a temperature above 25 °C ascribed to the higher DNA hybridisation reaction rate occurred between immobilised DNA probe and cDNA to form the duplex DNA at high temperatures.

Meanwhile, the DNA hybridisation time was determined by permitting the DNA hybridisation reaction to occur between 15 and 180 min, and the DNA biosensor response was recorded every 15 30 min.

DNA hybridisation time, on the other hand, is the rate limiting step, which determines the response time of the DNA biosensor.