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In the temporal profiles, increasing fluorescence intensity reflects FAs undergoing assembly, while decreasing intensity indicates disassembly.
Again these cells could be grouped according to increasing fluorescence intensity and each group could be analysed individually for viability.
In a histogram plot (Fig. 1), the presence of one bead per cell is indicated by the first positive peak (gate P5), and increasing numbers of beads (2 beads and ≥3 beads) are evidenced by successive peaks of increasing fluorescence intensity (gates P6 and P7, respectively).
Anisotropy values were found to increase exponentially with increasing fluorescence intensity (Fig. 2).
As can be seen, with increasing irradiation time, increasingly more hidden toehold was released, as demonstrated by the increasing fluorescence intensity after toehold-mediated DNA displacement reaction.
With each cycle of amplification, additional fluorophores are liberated from their probes and the increasing fluorescence intensity is monitored during the extension step of each PCR cycle.
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After approximately three hours of cultivation, FluBO expression was autoinduced as indicated by rapidly increasing fluorescence intensities of both FP domains.
As shown in Figure 5(a), when the N/P ratio of PEI-LA/LOR-2501 PEI-LA/LOR-2501 PEI-LA/LOR-2501bited markedly incomplexesluorescence intensity relative to those treated wash fluorescent-free ASO.
Blocking of the CD44 receptor decreased fluorescence intensity of cells, and GSH addition increased fluorescence intensity of cells again.
In most cases, the hydroxymethyl group increased fluorescence intensity even more than the aryl substituent, therefore it acted as enhancer.
The ensuing silica-coated, dye nanorods exhibited increased fluorescence intensity, non-leachability in polar solvents and high photostability.
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