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Although the backbones did not contain any TFS-like sequences, we again observed an increase in the relaxed conformation when the incubation time with TFO−C141S was increasing (data not shown).
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After 12 h post-induction, the productivity of secreted ATH35L for dsbAss did not increase (data not shown).
In consistent with our previous study (Zhang et al., 2002), the global ROS levels in T cells isolated from HHcy mice were increased (data not shown).
When BSMC were cultured, we confirmed that the cell density was increased (data not shown).
The total cell number of CD8+ T cells in these mice was only slightly but not significantly increased (data not shown).
When high concentrations of exogenous IL-2 or IL-7 were added, the proliferation of Teff cells increased (data not shown).
Interestingly, GFAP (glial fibrillary associated protein) positive cells were slightly increased (data not shown), while PSA-NCAM (polysialylated neural cell adhesion molecule) positive cells were dramatically increased (Figures S6A, S6B) at P31.
While IL-1β and IL-18 were also increased (data not shown), the expression of IL-33 was more than ten fold higher than either of these related cytokines.
Thymosin β-4, a protein implicated in the activation and mobilization of adult epicardial cells [9], and Thymosin β-10, a potent inhibitor of angiogenesis [23], were both slightly increased (data not shown).
Ligand affinity blot analyses showed adverse effects on laminin binding by all recombinant Len proteins when the ionic strength of the TBS-T buffer was increased (data not shown).
Metastasis size was also increased (data not shown).
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