The residual contrast, measured on shadow images of a 300 mesh grid, was >80% after corrections for increased dark current, following irradiation with up to 5×107 electrons/pixel (equivalent to 80,000 electron/μm2).
Mg-deficient leaves displayed increased dark respiration and decreased concentration of total soluble proteins, while Mg-deficient roots had decreased respiration and unchanged concentration of total soluble proteins.
This agrees with our data that Mg-deficient leaves had increased dark respiration, levels of SDH and ACO (Table 1), and decreased CO2 assimilation and stomatal conductance.
This phenotype, resulting from increased dark cycle locomotor bout frequency represents an immune effect on action selection at the basal ganglia.
In LD, females showed reduced transit and increased dark box duration, but males did not show any differences compared to corresponding sex of B6.
They also reported increased dark respiration and postulated that it was a mechanism to increase starch degradation at night, which prevented excess starch accumulation in leaves at dawn [ 48].
In contrast, Den Hertog et al. (1993) found increased dark respiration of the shoot but observed no change in root respiration in Plantago major when the carbon source was increased by raising the CO2 concentration.
This means that ATP synthesis catalyzed by ATP synthase in Mg-deficient leaves might be decreased, which might provide an advantage to prevent energy surplus due to increased dark respiration and the generation of excess reducing equivalents in chloroplasts.
These phenotypes included increased dark hypocotyl length in ddb1a and ddb1a ddb1b-2/+, accelerated flowering in long-day conditions in terms of leaf number in all lines, late flowering in long-day conditions in terms of days in ddb1b-2 ddb1a/+, decreased height in ddb1a ddb1b-2/+, and decreased silique length in both ddb1a ddb1b-2/+ and ddb1b-2 ddb1a/+.
