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3q22) in the regression on TIBC, indicating that increasing values of TIBC are associated with increasing copies of the minor allele in the rs3811647 genotype.
A positive linear regression coefficient indicates that increasing values of the quantitative outcome are associated with increasing copies of the minor allele in the genotype.
22q11) on TIBC, the parameter estimate was negative, indicating that decreasing values of TIBC are associated with increasing copies of the minor allele.
We observed association (additive model) between DKK1 SNP rs1569198 and dehydroepiandrosterone sulfate (DHEAS), with increasing copies of the A allele correlating with increasing DHEAS (AA: 2229.0 (1551.3), AG: 2099.0 (1373.8), GG: 1978.0 (1520.0) nmol/l; P = 0.05).
Illustrating a protective effect, increasing copies of the minor allele in the C282Y genotype were associated with decreasing values of TIBC and UIBC in the GWAS (observed P<3.15×10−7 for both), the VA follow-up study (observed P<0.04 for both), and the combined sample (observed P<3.0×10−9 for both).
This explains the increasing copies of kelch motifs ACBPs in B. rapa and B. oleracea (4 copies each).
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This novel mechanism for increasing copy number may have more widespread applications than the commonly used pUC high-copy origin of replication mutation.
We first established a standard curve by using increasing copy numbers (5 to 5×108 copies) of the plasmid Oxa-51/pCRII-TOPO in the assay (r = 0.9992, Text S3).
These results indicate a dose-dependent partial rescue with increasing copy numbers of His-GU transgenes.
Clustering decreased with increasing copy number; copy numbers 21 and 22, which included large outbreaks, were the exceptions.
Tandem duplication is known to be the most frequent mechanism in increasing copy numbers for gene families [ 32].
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