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Because this fraction increases with passage [4], deletion variants generally become a larger portion of the total virus population more rapidly when viruses contain larger inserts.
Our analysis of all markers is consistent with these observations, indicating that senescence gradually increases with passage.
TGFB1 and TGFB2 are secreted as latent complexes with the TGFβ binding proteins, LTBP1 and LTBP2 [ 136, 137], whose expression also increases with passage.
Senescence-associated β-galactosidase is one of the markers widely used to indicate replicative senescence since its activity increases with passage of time [ 5].
We previously showed that in non-immortalized cultures, p16-positive cells are continuously generated at low levels even at early passage, that this process increases with passage, and is independent of telomere dysfunction and p53-p21 pathway signaling [ 15].
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This indicates that not only do the Sca-1+-enriched passages still mainly consist of Sca-1+ cells, but the purity of Sca-1+ cells increases with passaging.
The proportion of these cells increases with passaging or following imposition of cellular stress at early passage (Passos et al., 2007).
In Pyongyang, official irritation with the United States increased with passage last month by the House of Representatives of the North Korean Human Rights Act, to support North Korean refugees in China.
In separate studies between 12.5% and 34% of hPSC lines were found to acquire chromosome abnormalities over time, with the incidence increasing with passage number.
Investigation of all biomarkers showed a gradual increase with passage with a plateau between passages 6-8.
SRPX, in contrast, did not correlate with SOX9 in the monolayer cultured HAC, and its expression increased with passage.
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