When the samples were left at ambient conditions for several weeks, the typical nanocluster size increased to approx. 330 400 nm and then remained stable.
Both A. digitata and A. gregorii withstood at least 5 and 4 min, respectively, without reaching 60 °C in the centre while surface temperature of the fruits further increased to approx. 300 °C.
The proportion of MMP-cliffs increased to approx. 25%.
Especially for IL-1β, the amount was increased to approx. 15-fold more than that in the joints of normal mice.
At 6 days after 3MC administration a >85% reduction in the expression of POR protein, determined both by immunoblotting and cytochrome c activity, had occurred, increasing to approx. 96% by day 14.
By 24 h, only the pO2 of the outer 500 µm of the bud had increased, up to approx. 15 kPa pO2 immediately within the scale, while the remaining path towards the core remained near levels seen in intact buds at 3 h (Fig. 3C).
As we described above, mechanical stimulation of control astrocytes caused a robust increase in [Ca2+]cyt, corresponding to approx. 3.8 μM.
Furthermore, since the NKA transports Na+ extracellularly, astrocytes treated with ouabain exhibited significant increase in [Na+]cyt, corresponding to approx. 24.1 mM, when compared with control.
Further increase in total activity to approx. 500 U L-1 was brought about by variation of cultivation parameters (IPTG concentration, induction time, temperature; data not shown) and by co-expression of pTf16 plasmid (coding for the trigger factor).
