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The correlation between the concentrated seawater color (brine) and viable and cultivable bacteria was previously investigated by Donadio et al [ 40] who reported that when the color intensity increases, the viable and cultivable bacteria concentrations, enumerated on plate count agar, increases.
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When the 30Kc6-expressing cells cultivated in the serum-containing medium were transferred directly to commercially available serum-free media, 30Kc6 expression increased the viable cell density by four-fold through inhibiting serum deprivation-induced apoptosis.
As for the prebiotics, inuline supplementation was reported to be able to increase the viable count of bifidobacteria in constipated elderly, while the frequency of the detection of enterobacteria decreased with the treatment.
This observation is in agreement with other studies where organic matter has been shown to increase the viable number of rhizobia and nodulation of peanut (Basu et al. 2008).
In a study by Bekatorou et al. (2001), freeze-dried S. cerevisiae cells immobilized on gluten pellets showed higher glycolytic activity concerning the fermentation time than free freeze-dried S. cerevisiae cells because immobilization increased the viable count of freeze-dried cells.
For the cells treated by 0.25 mM of H2O2, SWT increased the viable cells from 56.20 to 73.30%.
For example, 4 mg/ml SWT increased the viable cells from 64.70 to 70.82% for the cells treated by 0.125 mM of H2O2.
Considering the varied effect of α-KG, we have previously studied its effect in the production of monoclonal antibody, wherein it was found that α-KG not only increased the viable cell density by scavenging ammonia but it also increased the initial monoclonal antibody production [ 6].
An intrinsic limitation of our approach is imposed by the potential increase of the viable space's geometric complexity, when the dimension of the parameter space also increases.
When HS was combined with either a penetrant (DMSO) and/or a non-penetrant CPA (sucrose), only combinations in presence of DMSO displayed an increase in the viable cell number in proliferation at day 7 and 14 after thawing (Table 1 and Fig. 3A,B), indicating that the presence of HS does not improve the cryopreservative effect of the studied CPA solutions.
The proliferation of bacterial cells on quinalphos occurred in MSM up to 18 h as reflected by an increase in the viable cell count (Table 1).
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