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The use of this insertion site, that increases the cleavage potential of this reporter enzyme, can improve the sensitivity and dynamic range of the antiviral drug assay.
A comparison of the rate of cleavage of X0n with and without a 5' phosphate shows that the presence of a 5' phosphate increases the cleavage rate by >50-fold (Figure 1E).
In vivo, Angptl8 increases the cleavage of Angptl3 [ 19], releasing its N-terminal domain, which has been shown to inhibit LPL activity [ 32].
The DExD/H-box domain might have an auto-inhibitory function, because removal of this domain increases the cleavage rate of the human dicer [ 14].
Furthermore, inhibition of the PI3K/Akt pathway increases the cleavage of caspase 8 in colon cancer cells treated with E-selectin and this effect is still further increased when both ERK and PI3K pathways are concomitantly inhibited.
We recently described that a single substitution at this site, T259I that is typical for ancestral Y. pestis lineages, increases the cleavage of the light chain of plasmin, thus decreasing the stability of the formed plasmin [ 26].
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Meanwhile, compared with the control group, HMGB1 3 siRNA significantly increased the cleavage of PARP (Fig. 5e) and apoptosis (Fig. 5c) and decreased the viability of SW982 cells (Fig. 5d).
The addition of T to IVM media increased the cleavage and blastocyst formation rates in plasticware and may be employed to improve the efficiency of the standard in vitro embryo production systems.
Supplementation of the IVC medium with 200 μM cysteamine significantly increased the cleavage rate and blastocyst yield, while the other concentrations (50, 100 and 400 μM) had no effect.
Activation of meprin by in situ trypsin treatment of cell cultures increased the cleavage of E-cadherin and resulted in the accumulation of the 97-kDa fragment at the expense of the 120-kDa mature form (Figure 1A).
This regulatory complex binds to the end of the 20S proteasome core, increasing the cleavage rate in an ATP-independent manner and affecting the quality of protein digestion [5], [8].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com