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Deletion of this enzyme increases TAG levels [12].
However, co-expression of both the Sco0958 and Lppβ enzymes increases TAG production by 1.6-fold.
In particular, while DosR deletion abolishes TAG production in hypoxia, DosR induction increases TAG production.
Our work extends previous studies in Drosophila that showed that excess dietary sugar increases TAG storage (Skorupa et al., 2008).
Furthermore, it has been recently described that pharmacological inhibition of autophagy increases TAG levels in hepatocytes, whereas autophagic inducers decrease their levels (Singh et al., 2009).
Figure 3B shows that the sole expression of SCO0958 from the strong T7 promoter notably increases TAG content in the cell respective to its expression from the PBAD promoter, demonstrating that under these conditions higher levels of the DGAT Sco0958 result in higher levels of TAG biosynthesis.
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Increased TAG levels were also associated with increased ALT (P = 0.031).
Collisions lead to losses in bandwidth and energy, and increase tag identification delays.
In addition, the work required to parse a sentence goes up with increasing tag ambiguity, though not as much as one might expect.
Metabolic engineering is one method to increase TAG yields, provided that appropriate gene target(s) are identified.
Overexpression of the enzyme resulted in significantly increased TAG accumulation without a negative effect on growth or biomass accumulation.
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