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This indicates that heterologous expression of the I- SceI nuclease increased transformation efficacy and transformant stability.
Prestrained thermoelastic martensite alloys have the characteristics of increased reverse transformation temperature and increased transformation heat by cold deformation.
We report here that treatment with LiAc and DDT before electroporation increased transformation efficiency to 225 ± 52.5 × 107 transformants per μg DNA, while with untreated cells or treated with LiAc alone transformation efficiency approximated 1.2 ± 0.5 × 105 transformants per μg DNA.
For example, MK and MX both induce P450-dependent oxygenases (Lehman-McKeeman et al. 1995; Mersch-Sundermann et al. 1996, 2001; Suter-Eichenberger et al. 1999) and therefore could cause increased transformation of other environmental chemicals that are mutagenic in their transformed form such as benzo(a pyrene, 2-aminoanthracene, and aflatoxin B1 (Mersch-Sundermann et al. 1996, 2001).
Since deletion of comI in B. licheniformis DSM13 resulted in twofold increased transformation efficiency by genetic competence and overexpression resulted in threefold decreased transformability, the function as a competence inhibitor became evident.
However, when their contents increased, transformation temperatures of Ar1 and Ar3 is decrease during RCR + ACC process.
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To determine whether a DSB mediated by I- SceI can increase transformation and recombination efficiency in T. reesei, the aforementioned reporter strain JP7.7.12 was transformed with a linear fragment comprising a glucoamylase expression cassette (Fig. 4).
Increasing transformation and fragmentation of rural landscapes for economic reasons is causing concern to conservationists worldwide.
In general, transformation was found to be dependent on the study temperature; increasing temperatures simultaneously result in increasing transformation rates.
TEM investigations support that slip in austenite occurs concomitant with increasing transformation strains.
The transformation from B2 austenite to B19′ martensite is suppressed by a transformation barrier that increases with increasing transformation strains and decreasing diameter of the nanocrystals.
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