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When the 30Kc6-expressing cells cultivated in the serum-containing medium were transferred directly to commercially available serum-free media, 30Kc6 expression increased the viable cell density by four-fold through inhibiting serum deprivation-induced apoptosis.
In a study by Bekatorou et al. (2001), freeze-dried S. cerevisiae cells immobilized on gluten pellets showed higher glycolytic activity concerning the fermentation time than free freeze-dried S. cerevisiae cells because immobilization increased the viable count of freeze-dried cells.
For the cells treated by 0.25 mM of H2O2, SWT increased the viable cells from 56.20 to 73.30%.
For example, 4 mg/ml SWT increased the viable cells from 64.70 to 70.82% for the cells treated by 0.125 mM of H2O2.
Considering the varied effect of α-KG, we have previously studied its effect in the production of monoclonal antibody, wherein it was found that α-KG not only increased the viable cell density by scavenging ammonia but it also increased the initial monoclonal antibody production [ 6].
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As for the prebiotics, inuline supplementation was reported to be able to increase the viable count of bifidobacteria in constipated elderly, while the frequency of the detection of enterobacteria decreased with the treatment.
This observation is in agreement with other studies where organic matter has been shown to increase the viable number of rhizobia and nodulation of peanut (Basu et al. 2008).
The correlation between the concentrated seawater color (brine) and viable and cultivable bacteria was previously investigated by Donadio et al [ 40] who reported that when the color intensity increases, the viable and cultivable bacteria concentrations, enumerated on plate count agar, increases.
Moreover, methylcellulose increased the rate of viable spermatozoa with intact acrosome and low lipid disorder, reducing the ROS generation.
Hypothermia (20 °C>15 °C>37 °C) and alkalinization (pH 9.3>pH 8.3>pH 7.4) increased the yield of viable neurons compared with the yield from DRG maintained under physiological conditions.
The differences in metabolic and redox imprints were further reflected in the transfection performance of the polycations, but co-treatment with the GSH precursor N-acetyl-cysteine (NAC) counteracted redox dysregulation and increased the number of viable transfected cells.
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