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Meanwhile, we improved the technology by optimizing the concentration of Cas9 and sgRNAs and designing two adjacent sgRNAs targeting one exon for each gene, which greatly increased the targeting efficiency and bi-allelic mutations.
Moreover, SUV420H1_i2 distribution was unaffected by co-expression of heterochromatin protein-1α, which increased the targeting of SUV420H1_i1 and SUV420H2 to regions of pericentromeric heterochromatin.
Similar to our previous observation that RbBP5 depletion led to the enrichment of CD8-CIMPR at cell protrusions [5], siRNAs against RbBP5 increased the targeting of internalized β1 integrin to these sites (Fig. 7C).
Furthermore, overexpression of roX2 (but not roX1 ) in females using roX transgenes under a hsp83 promoter increased the targeting frequency.
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It could also increase the targeting and efficacy of vaccines at low doses.
The successful containment provides evidence for consideration of the use of foam to improve traditional flushing techniques, by increasing the targeting of contaminants by remedial agents.
Indeed, the integral binding avidity plays a predominant role in the targeting efficacy; the higher integral binding avidity increases the targeting efficacy.
In the presence of Mg2+, addition of dNTPs did not significantly increase the targeting efficiency (not shown).
siRNAs used in the screen were designed using an algorithm developed to increase the targeting efficiency of the siRNA while reducing off-target silencing [35].
Different methods, using mainly viral and nonviral vehicles, have been developed to increase the targeting and efficiency of miRNAs.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com