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We performed SELEX with the purified, cross-linked Aβ40 trimers using filter binding, and increased the stringency of the selection gradually during the process (see Materials and Methods).
We therefore increased the stringency of the selection process to enrich for specific peptide sequences during the 2nd selection by shortening the binding time from 2 h to 5 min and increasing the number of washes from 3 to 15.
We introduced sublibrary 9.1, 3p, and a random control shRNA into FL5.12 cells by retroviral infection, and subjected the cells to cycles of IL3 "starvation and recovery" just as with the original library except that we increased the stringency of selection by extending the length of the IL3 starvations.
These two options increased the stringency in sequence alignment.
We therefore increased the stringency of cross-linking by reducing the fixation temperature on ice.
These criteria increased the stringency of our counts, and helped minimize double counts.
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The bill will also increase the stringency and frequency of safety inspections for food manufacturers and processors.
The use of this parameter increases the stringency for reads being assembled together (Chapman 2015).
Some Ugandan media reports even suggest that the incentive of tourism dollars could increase the stringency with which this tradition is enforced by, for example, offering rewards for the return of reluctant men living outside the region.
"The EU has a very long-standing process of increasing the stringency of emission standards from vehicles," says Ekins. "That in itself means nothing at all, unless those standards are enforced".
Increasing the stringency from 2 to 6 peptides resulted in 1504 identified proteins composed of 129 core proteins (8.6%).
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