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We increased the imaging depth by stacking layers of chicken breast tissue.
Data taken at 100 frames/s, we increased the imaging speed to 400 Hz at 1 mM ATP concentration.
For in vivo imaging, we also intentionally increased the imaging depth by placing ~2 cm thick chicken tissue atop the rat.
Fourier domain OCT (FD-OCT), including swept source OCT (SS-OCT) and spectral domain OCT (SD-OCT), has significantly increased the imaging speed and minimized the motion artifacts [ 7, 22– 25].
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For modern scanners with pitch-corrected mAs, this does not result in a dose penalty but does increase the imaging time (which can lead to image degradation due to movement).
To increase the imaging resolution at ×40 magnification, 24-well glass bottom well plates were used (PAA Laboratories GmbH, Austria).
It can efficiently increase the imaging gray levels and speed up frame frequency of display systems.
Recently, multi-slice and 3D VASO techniques have been implemented to increase the imaging volume beyond the single slice of early reports.
Although this approach satisfied the objective of increasing the imaging speed, it required an instrument that is more complex, expensive, and difficult to maintain.
It should also be noted that with a tilted gantry spiral acquisition is not possible, which increases the imaging time and decreases the quality of reformats.
In order to increase the imaging speed of a scanning probe microscope in tapping mode, we propose to use a dynamic controller on 'parachuting' regions.
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