Exact(5)
Analysis of the human HBB poly(A) region showed a similar trend with increased termination correlating with increased sequence length (Figure 2B).
Dell's latest bid, which was made privately and disclosed by 3Par, included an increased termination fee of $92 million as well as a multiyear reseller agreement if 3Par accepted a different offer, 3Par said.
The increased termination at more downstream position leaves only the faster polymerase populations and the apparent elongation rates are higher.
To determine if part of the increased termination was simply due to the increased template length, we inserted several kilobases of heterologous coding sequence downstream of each poly(A) region test fragment.
Abnormally long pauses and slow polymerization were generally correlated with increased termination and decreased pause times, whereas fast elongation was associated with decreased termination.
Similar(55)
RNA-binding proteins can affect termination at sequences both upstream and downstream of the TEC, either by stabilizing the hairpin structure to increase termination efficiency, or by disrupting this structure and acting as anti-terminators [41], [42].
While we had thought that addition of a length of heterologous sequence downstream of the polyadenylation signals would increase termination, the reverse was true.
These effects were exacerbated when bridged H-NS was present, inhibiting overall elongation and increasing termination relative to samples containing only Rho or both NusG and Rho.
Because small changes in effects on transcript termination are cumulative, unlike the switch-like effects that operate during initiation, modest changes in the amounts of bridging could be magnified (e.g., increasing termination from 1%to10%0% at 20 sites in an operon will reduce expression by a factor of 7).
Valeant also increased its termination fee to $4.5 million Canadian from $3.75 million Canadian.
The result indicates that the buffer is able to significantly reduce the SU blocking probability and noncompletion probability with very minor increased forced termination probability.
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