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Guide strands were selected for favorable strand biasing, but increased strand biasing did not correlate with potency, suggesting a threshold effect.
This behaviour, rather similar to the known influence of ion content on tensile strength, is attributed to the importance of a growing ionic cluster phase that leads to an increased strand density.
The resolution of the most complex high GC-rich repeats was achieved by sequencing of PCR products amplified with a hybrid TopoTaq DNA polymerase with increased strand displacement capacity.
Similar(57)
Based on previous data, increased strand-displacement activity could also be a feature of the exonuclease-deficient Polγ.
With this assumption, the shared similarities could result from the formation of longer DNA flaps by increased strand-displacement by the exonuclease-deficient Polγ, or by defects in the removal of DNA flaps in the case of MGME1 deficiency.
A general feature of exonuclease-deficient DNA polymerases is an increased strand-displacement activity, as has been observed for T7 DNA polymerase (59), T4 DNA polymerase (60), Escherichia coli DNA polymerase II (61) and yeast Polδ (62).
This decrement becomes more noticeable with increasing cover and decreasing concrete tensile strength, but shows no remarkable changes with an increasing strand diameter and rust expansion ratio.
The average bond strength of prestressing strands embedded in UHPFRC decreased with increasing strand diameter.
From the above discussion, the following conclusions are drawn: The average bond strength of prestressing strands embedded in UHPFRC decreased with increasing strand diameter.
F61 mutations have been shown to increase fidelity of nucleotide incorporation [36], [37], reduce the ability of RT to extend a mismatched primer terminus [36], decrease processivity [37], [38], either decrease or increase strand displacement activity [38], [39], and enhance the ability to bypass lesions in the template strand [18].
In this step, Actinomycin D was used to increase strand specificity by inhibiting second-strand cDNA synthesis.
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