Exact(4)
These combined effects resulted in the favorable sensitivity and high selectivity to H2, and also contributed to the increased stability at the elevated sensing temperatures.
The Arg variant (Oluc-N166R) was most improved (∼50% increased stability at 37 °C and ∼3-fold higher luminescence intensity) and was used as the template for directed evolution.
Another noteworthy characteristic of MnP4 is the high number of surface-exposed lysines (up to a total of 20) and other alkaline residues (up to a total of 35) compared with the other P. ostreatus PODs, which would result in a higher net positive charge of the protein and increased stability at acidic pH.
There is also evidence for subshells (regions of somewhat increased stability) at Z = 108 and N = 162.
Similar(56)
Mutations of functional residues usually decrease enzyme activity but often increase stability at the same time [32], [33].
Interestingly, at faster speeds midleg AEP and PEP are also shifted laterally, perhaps to increase stability at higher speeds.
Simple proprioceptive drills to help increase stability at the lumbar spine, hip, knee, and shoulder were presented and participants were encouraged to utilize the drills regularly.
The increased stability of Cx43 at the plasma membrane seems to be specific and not related to disruption of endocytic pathway, as demonstrated by dextran uptake.
The increased stability of theaflavins at neutral pH could make these black tea compounds a more feasible option for the design of an antiviral therapeutic agent than EGCG.
Data further suggests that increased intercellular communication results from increased stability of Cx43 at the plasma membrane, presumably forming functional gap-junctions, as suggested by decreased solubility of Cx43 in 1% Triton X-100.
Results demonstrate, for the first time, that 7-keto induces an increase in gap junction intercellular communication, that is most likely due to an increased stability of protein at the plasma membrane and to increased abundance of Cx43 assembled in gap junction plaques.
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