Similar inhibitor specificity profiles correlate with increased sequence homology at specific alignment positions.
Longer sequences will have better correlation properties, but increased sequence length leads to more time needed for synchronization.
As expected, the frequency of Mendelian errors diminishes as a function of increased sequence depth.
Out of 184 segmental duplications from the target region of HSA21 we observe increased sequence coverage for 95 outside the target region.
Our data support these findings, and further demonstrate that a common feature of the breakpoints, irrespective of the type, is a significantly increased sequence homology around the breakpoint.
At 75d, the distribution of percent changes is shifted towards negative values, reflecting increased sequence variability in the UD-UD condition (Fig. 5 E).
Therefore, we created a new dataset with greatly increased sequence data by aligning the genomes of the 13 strains for which genome sequencing has either been completed or is near completion, giving 1.5 Mb of aligned sequence (Figure 1).
Local anomalous DNA conformation attributable to the Alu-Yi6 element, as suggested by enhanced DNase I sensitivity and obstruction to DNA sequencing, could be a contributing factor of the increased sequence diversity.
The digest was done in the presence of a mass spectrometry friendly surfactant (Protease Max, Promega) to provide increased sequence coverage and the reduction and alkylation steps were excluded.
It is possible that the increased sequence diversity in the early epidemic was somehow related to non-neutral drift during that period, perhaps across larger populations than we have investigated.
As expected, consistent NFR segments showed increased sequence conservation.
